Table 1

Sensitivity and specificity values for qPCR calculated using HEYM culture as a gold standard
Shedding threshold for qPCR test positivity (organisms/g)
10-1 101 102 103 104 105 106
Sens. 0.91 0.91 0.91 0.85 0.68 0.52 0.37
Spec. 0.73 0.73 0.83 0.89 1.00 1.00 1.00
PPV 0.91 0.91 0.94 0.95 1.00 1.00 1.00
NPV 0.74 0.74 0.75 0.66 0.52 0.42 0.35

Sensitivity and specificity values for qPCR calculated for different shedding states using HEY solid medium as a gold standard based on NVSL proficiency panel culture results.

O’Brien et al.

O’Brien et al. BMC Veterinary Research 2013 9:72   doi:10.1186/1746-6148-9-72

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