Table 5

Results of testing oro-pharyngeal swabs for Newcastle disease virus by number of swabs pooled
Swab source Swab pool 1 DPI 2 DPI 3 DPI 4 DPI 7 DPI
VI rRT-PCR VI rRT-PCR VI rRT-PCR VI rRT-PCR VI rRT-PCR
oro-pharyngeal 1 swab 20/20 (100) 20/20 (100) 20/20 (100) 20/20 (100) 20/20 (100) 20/20 (100) 20/20 (100) 20/20 (100) 1/20 (5) 14/20 (70)
5 swabs 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 1/38 (2.6) 23/38 (60.5)
11 swabs 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 38/38 (100) 2/38 (5.3) 16/38 (42.1)
cloacal 1 swab 2/20 (10) 7/20 (35) 2/20 (10) 4/20 (20) 12/20 (60) 9/20 (45) 11/20 (55) 13/20 (65) 11/20 (55) 10/20 (50)
5 swabs 10/38 (26.3) 7/38 (18.4) 3/38 (7.9) 10/38(26.3) 19/38 (50) 28/38 (73.7) 23/38 (60.5) 17/38 (44.7) 15/38 (39.5) 15/38 (39.5)
11 swabs 8/38 (21.1) 9/38 (23.7) 8/38 (21.1) 11/38 (28.9) 17/38 (44.7) 15/38 (39.5) 27/38 (71.0) 20/38 (52.6) 16/38 (42.1) 17/38 (44.7)

Non-flocked swabs were collected in brain heart infusion broth and were tested by virus isolation and real-time RT-PCR. There were no statistical differences based on the number of swabs pooled. Abbreviations: DPI = days post inoculation, rRT-PCR = real-time RT-PCR, VI = virus isolation.

*. Number positive/total tested (percent positive).

Spackman et al.

Spackman et al. BMC Veterinary Research 2013 9:35   doi:10.1186/1746-6148-9-35

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