Table 3

Results of testing oro-pharyngeal swabs for avian influenza virus by transport condition
Transport media Swab removed prior to transport Number of swabs per vial 2 DPI 3 DPI 4 DPI 7 DPI
VI rRT-PCR VI rRT-PCR VI rRT-PCR VI rRT-PCR
3.5 ml BHI No 1 10/10 (100)*a 15/15 (100)a 10/10 (100)a 15/15 (100)a 10/10 (100)a 15/15 (100)a 2/10 (20)a 2/15 (13.3)a
3.5 ml BHI No 5 10/10 (100)a 15/15 (100)a 10/10 (100)a 15/15 (100)a 10/10 (100)a 15/15 (100)a 2/10 (20)a 0/15 (0)a
3.5 ml BHI Yes 1 10/10 (100)a 15/15 (100)a 9/10 (90)a 14/15 (93.3)a 10/10 (100)a 15/15 (100)a 4/10 (40)a 0/15 (0)a
3.5 ml BHI Yes 5 10/10 (100)a 14/15 (93.3)a 10/10 (100)a 15/15 (100)a 10/10 (100)a 15/15 (100)a 2/10 (20)a 0/15 (0)a
2 ml BHI No 1 10/10 (100)a 9/10 (90)a 10/10 (100)a 10/10 (100)a 10/10 (100)a 10/10 (100)a 0/10 (0)a 0/10 (0)a
2 ml BHI Yes 1 10/10 (100)a 10/10 (100)a 9/10 (90)a 9/10 (90)a 10/10 (100)a 10/10 (100)a 0/10 (0)a 0/10 (0)a
None No 1 8/10 (80)a 12/15 (80)a 6/10 (60)ab 15/15 (100)a 7/10 (70)a 14/15 (93.3)a 0/10 (0)a 0/15 (0)a
None No 5 10/10 (100)a 14/15 (93.3)a 4/10 (40)b 14/15 (93.3)a 6/10 (60)a 14/15 (93.3)a 0/10 (0)a 0/15 (0)a

Flocked swabs and brain heart infusions broth were used for all sample collection except dry transport where culturettes with self-contained swabs were used. Statistical groups (within each detection test [virus isolation or rRT-PCR] and day post inoculation) are denoted with letters, where groups which do not share a letter have a statistically significant difference with a p of value < 0.05. Abbreviations: BHI = brain heart infusion broth, DPI = days post inoculation, rRT-PCR = real-time RT-PCR, VI = virus isolation.

*. Number positive/total tested (percent positive).

Spackman et al.

Spackman et al. BMC Veterinary Research 2013 9:35   doi:10.1186/1746-6148-9-35

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