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Open Access Highly Accessed Research article

Isolation of mycobacteria from clinical samples collected in the United States from 2004 to 2011

Tyler C Thacker1*, Suelee Robbe-Austerman2, Beth Harris2, Mitchell Van Palmer1 and Wade Ray Waters1

Author Affiliations

1 United States Department of Agriculture, Infectious Bacterial Disease Research Unit, National Animal Disease Center, Agricultural Research Service, Ames, IA, USA

2 United States Department of Agriculture, Mycobacteria and Brucella Section, National Veterinary Services Laboratories, Ames, IA, USA

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BMC Veterinary Research 2013, 9:100  doi:10.1186/1746-6148-9-100

Published: 8 May 2013

Abstract

Background

Mycobacteria other than M. bovis may interfere with current bovine tuberculosis diagnostic tests resulting in false positive test results. As the prevalence of M. bovis decreases in the United States, interference from other mycobacteria play an increasingly important role in preventing the eradication of M. bovis. To identify mycobacteria other than M. bovis that may be interfering with current diagnostic tests, a retrospective study was performed to identify mycobacteria isolated from clinical tissues at the National Veterinary Services Laboratories between 1 January 2004 and 9 October 2011.

Results

During the study period, 2,366 mycobacteria other than M. bovis were isolated from samples submitted for clinical diagnosis of M. bovis. Fifty-five mycobacterial species were isolated during this time period. In cattle, M. avium complex, M. fortuitum/fortuitum complex, M. smegmatis, M. kansasii, and M. terrae complex were the predominate species other than M. bovis isolated from tissues submitted for culture. Mycobacteria other than M. bovis isolated from deer were predominantly M. avium complex, M. terrae/terrae complex, and M. fortuitum/fortuitum complex.

Conclusions

These data provide information characterizing the species and relative prevalence of mycobacteria other than M. bovis that may interfere with current diagnostic tests.