Agarose gel electrophoretic analysis showing the efficiency of various PCR additives in the amplification of GC rich DNA templates. The additives were DMSO (7.5%), Propan-1, 2-diol (PNL) (0.816 M), and Ethylene glycol (EG) (1.075 M). Numbers 1, 2, 3 represent three different MAP isolates which were difficult templates to amplify, while 4 represents K10 strain. L: 100 bp DNA marker. On the left (A) are PCR products from amplification of SSR locus 1, while on the right (B) are products of SSR locus 2. Best results were obtained using Ethylene glycol.
Okuni et al. BMC Veterinary Research 2012 8:99 doi:10.1186/1746-6148-8-99