Table 2

Down- or up-regulated proteins after 72 hours masitinib treatment
Spot. Identified protein AV t test accession no. in NCBI MW [Da]2 pI§ MOWSE score assigned peptides sequ. cov. [%]
Down-regulated proteins
1 TARDBP43 −2.62 0.019 gi|6678271 (Ho) 45053 5.85 53 1* 4*
6 EIF4A −1.66 0.005 gi|73966207 (Ca) 44692 5.40 89 15 41
7 TCP1A −1.63 0.019 gi|73945755 (Ca) 60943 5.71 80 9 17
2 EIF3 −1.33 0.012 gi|73974369 (Ca) 29504 7.78 92 8 21
8 PPA1 −1.25 0.021 gi|73953384 (Ca) 33372 5.80 34 1* 2*
9 CCT2 −1.25 0.042 gi|73968673 (Ca) 57737 6.01 80 8 17
3 ACTR2 −1.12 0.019 gi|73969816 (Ca) 36488 8.21 46 1* 4*
10 PSMD6 −1.06 0.040 gi|73981961 (Ca) 99123 9.57 72 10 12
Up-regulated proteins
11 HSP90B1 +1.18 0.048 gi|50979116 (Ca) 22925 6.23 74 5 24
11 GSTT3 +1.20 0.0077 gi|73995693 (Ca) 27793 6.67 98 8 33
12 YWHAZ. +1.22 0.019 gi|73974186 (Ca) 26438 5.00 156 17 52
13 PDIA3 +1.26 0.012 gi|74000349 (Ca) 76474 8.95 161 21 30
14 CORO1A +1.34 0.017 gi|73958506 (Ca) 41955 7.10 52 1* 2*
15 TTC38 +1.34 0.016 gi|73968897 (Ca) 52886 5.67 79 6 12
16 OXCT1 +1.43 0.028 gi|73954281 (Ca) 65275 7.92 90 10 18
17 SEMG1 +1.45 0.017 gi|134093092 (Go) 52065 8.96 83 8 19
18 UROD +1.45 0.016 gi|73977144 (Ca) 35096 5.50 105 9 29
19 ANXA6 +1.50 0.031 gi|73953627 (Ca) 76241 5.47 207 23 32
20 UCHL3 +1.51 0.027 gi|158749588 (Ra) 26278 5.01 74 5 23
21 SELENBP1 +1.65 0.0011 gi|73981582 (Ca) 67142 7.59 89 9 20
22 IRX6 +1.74 0.0018 gi|121484129 (Pa) 24682 8.99 74 5 24
5 CAPG +1.98 0.0036 gi|73980918 (Ca) 39088 6.04 85 6 24
4 ANXA1 +2.58 0.007 gi|73946797 (Ca) 38887 5.84 91 12 31

§The listed molecular weights and pI values correspond to the listed accession numbers, which sometimes belong to species other than Canis familiaris (Ca).

*These proteins were identified by unique peptide sequences determined by MALDI-TOF MS/MS experiments.

Abbreviations: EIF4A: eukaryotic translation initiation factor 4A; TCP-1-alpha: T-complex protein 1, alpha subunit; EIF3: eukaryotic translation initiation factor 3; ACTR2: actin-related protein 2; EIF3: eukaryotic translation initiation factor 3; HSP-ß1: heat shock protein beta-1; PDI: protein disulfide isomerase-associated 3 precursor; CORO-1A: coronin-1A; UPD: Uroporphyrinogen decarboxylase; ANXA6: annexin A6; MCP: Macrophage capping protein; ANXA1: annexin A1; Ca: Canis familiaris; Ho: Homo sapiens; Pan: Pan troglodytes; Ra: Rattus; Go: Gorilla.

Klopfleisch et al.

Klopfleisch et al. BMC Veterinary Research 2012 8:96   doi:10.1186/1746-6148-8-96

Open Data