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Open Access Research article

Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina

Silvina S Maidana13, Patricia M Lomonaco1, Gustavo Combessies5, María I Craig13, Julian Diodati1, Daniela Rodriguez1, Viviana Parreño1, Osvaldo Zabal1, José L Konrad2, Gustavo Crudelli2, Axel Mauroy4, Etienne Thiry4* and Sonia A Romera13

Author Affiliations

1 Instituto de Virología, Centro de Investigaciones en Ciencias veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25 (B1712WAA), Buenos Aires, Castelar, Argentina

2 Facultad de Veterinaria, Universidad Nacional del Nordeste, 25 de Mayo 868, Corrientes 3400, Argentina

3 Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917 (C1033AAJ), Ciudad Autónoma de Buenos Aires, Argentina

4 Veterinary Virology and Animal Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster, 20, B43b, Liège, B-4000, Belgium

5 Laboratorio Azul, Av., 25 de Mayo 479/485 (7300), Azul, Buenos Aires, Argentina

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BMC Veterinary Research 2012, 8:83  doi:10.1186/1746-6148-8-83

Published: 20 June 2012

Abstract

Background

Parainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions.

Results

Examination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c).

Conclusions

This is the first characterization of BPIV3 in water buffalo.

According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C were found in cattle.