Single-step growth curves of wild virus and recombinant viruses. BHK-21 cells were infected with wild virus and recombinant viruses at MOI of 0.1 TCID50 per cell. At several time points, cells were harvested and the titers of the viruses were determined with TCID50 per milliliter using Reed–Muench formula.
Li et al. BMC Veterinary Research 2012 8:57 doi:10.1186/1746-6148-8-57