Table 1

Virus neutralization test (VNT) results on samples from reindeer (Rangifer tarandus tarandus) and caribou (Rangifer tarandus granti) from Alaska.

Caribou

Reindeer


Mean ED50 titre1

Mean ED50 titre1


BoHV1

9,4

31,0


CvHV1

13,0

29,3


CvHV2

131,0

136,7


ElkHV1

31,0

55,1


Neutralizing titers were calculated according to the Spearman-Kärber method as the serum dilution necessary to neutralize the cytopathic effect in 50% of the wells (effective dose 50%; ED50) and are presented as the mean values for each virus titration (samples classified as negative in ELISA are excluded). Virus back titrations for the reindeer and caribou neutralizations oscillated between 90 and 115 TCID50, (90 for CvHV1, 95 for BoHV1, 110 for ElkHV and 115 for CvHV2).

Evans et al. BMC Veterinary Research 2012 8:5   doi:10.1186/1746-6148-8-5

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