|Efficiencies of the totalMycobacterium aviumsubsp.paratuberculosisDNA isolation procedure from the faeces|
|Theoretical input of Map cells per g faecesa||Experimental mean output of Map cells per g faecesb||Mean DNA isolation efficiency (%)c|
Map, Mycobacterium avium subsp. paratuberculosis.
aDetermined by OD600 of bacterial suspension.
bCalculated as the mean of the recovered IS900 copy number from IS900 qPCR divided by 15, considering there is on average 15 IS900 copies per single Map cell.
cCalculated as the mean yield obtained using HYDEqPCR divided by the theoretical number of Map cells added into the faeces, multiplied by 100.
Logar et al.
Logar et al. BMC Veterinary Research 2012 8:49 doi:10.1186/1746-6148-8-49