## Table 1 |
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Efficiencies of the totalMycobacterium aviumsubsp.paratuberculosisDNA isolation procedure from the faeces |
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Theoretical input of Map cells per g faeces^{a} |
Experimental mean output of Map cells per g faeces^{b} |
Mean DNA isolation efficiency (%)^{c} |

900,000 | 586,667 | 65 ±38 |

90,000 | 82,833 | 92 ±16 |

9,000 | 6,747 | 76 ±3 |

900 | 704 | 78 ±19 |

90 | 100 | 112 ±30 |

Map, *Mycobacterium avium* subsp. *paratuberculosis.*

^{a}Determined by OD_{600} of bacterial suspension.

^{b}Calculated as the mean of the recovered IS*900* copy number from IS*900* qPCR divided by 15, considering there is on average 15 IS*900* copies per single Map cell.

^{c}Calculated as the mean yield obtained using HYDEqPCR divided by the theoretical number
of Map cells added into the faeces, multiplied by 100.

Logar * et al.*

Logar * et al.* *BMC Veterinary Research* 2012 **8**:49 doi:10.1186/1746-6148-8-49