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Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA

Katarina Logar*, Rok Kopinč, Petra Bandelj, Jože Starič, Aleš Lapanje and Matjaž Ocepek

BMC Veterinary Research 2012, 8:49  doi:10.1186/1746-6148-8-49