Immunohistochemical micrographs of different tissues of Atlantic cod. (a) Liver: all the hepatocyte cytoplasm and tissue that form cellular network were Pis1-immunoreactive. (b) Bile duct and (c) Gall bladder epithelium were Pis1-immunoreactive. (d) The tissue of bulbus arteriosus: numerous elongated fibrocytes and tissues that form cellular network were Pis1-immunoreactive. (e) Peritoneum: tissues that form cellular network lining a layer of mesothelium (*) were Pis1-immunostained. (f) Hypodermis layer (Hm) of the skin and muscle fibers (Mf): cells and connective tissues showing Pis1-positivity (g) Oocytes: various stages of the cells showing Pis1-immunoreactivity particularly the early or mid vitellogenic stage (*) exhibited strong positive signals. Negative control (inset) of the serial sections treated with dilution buffer was Pis1-immunonegative (Larger images are seen in Additional file 1: Figure S6).
Ruangsri et al. BMC Veterinary Research 2012 8:46 doi:10.1186/1746-6148-8-46