(5A) Immunohistochemical micrographs of different neural tissues (including eye) of Atlantic cod. (a) Parasympathetic ganglia: various sized neuronal cell bodies (arrows), cells and tissues in axons and dendrites (*) were Pis1-immunoreactive (Hk: head kidney’s hematopoietic tissue). (b) Eye: Pis1-immunoreactivity was found in various cell types and tissues –including 1: sensory cells of the retina; 2: cells and tissues of inner nuclear layer; 3: inner plexiform layer; 4: ganglion cell and nerve fiber layer, and arrowhead shows the inner limiting membrane. The chondrocytes of the hyaline cartilage (arrows), connective tissues (Ct), and tissue of the lens (*) were also immunoreactive, (m: melanocytes). (5B) Section of exocrine and endocrine glands of Atlantic cod. (a) Exocrine pancreas: the cords of exocrine acinar cells (*) showing strong intensity of Pis1-immunoreactivity; note the presence of the large hepatic veins (arrows). (b) Corpuscles of Stannius: Pis1-immunoreactivity was found in the cytoplasm of most secretory cells. Negative control (inset) of the serial sections treated with dilution buffer was Pis1-immunonegative. (Larger images are seen in Additional file 1: Figure S5).
Ruangsri et al. BMC Veterinary Research 2012 8:46 doi:10.1186/1746-6148-8-46