Immunohistochemical micrographs of different tissues of Atlantic cod. (a) Dorsal skin: numerous epithelial cells were Pis1-immunoreactive, but the basal cells (arrows) exhibited stronger immunoreactivity than the apical cells (Gb: goblet cell; Sc: saccular cell). (b) Epidermal layer of skin surrounding the eye - some Pis1-immunoreactivity was observed in the cytoplasm of epithelial cells (arrows), (N: cells of neuromast). (c) Gill filament: multi-granular cells of primary lamella (arrows) and epithelial cells of secondary lamella (arrow heads) were Pis1-immunoreactive. (d) Adductor muscle: multi-granular cells adjacent to the muscle fibers (Mf) were Pis1-immunostained. (e) Cartilaginous tissue of the primary lamella: all the chondrocytes (*) exhibited very strong Pis1-immunoreactivity. (f) Cartilaginous tissue of the gill filament: only chondrocytes (open arrows) were Pis1-immunostained (positive). Negative control (inset) of the samples treated in dilution buffer was Pis1-immunonegative (Larger images are seen in Additional file 1: Figure S3).
Ruangsri et al. BMC Veterinary Research 2012 8:46 doi:10.1186/1746-6148-8-46