Immunohistochemistry on Atlantic cod leucocytes and different hematopoietic tissues. (a) The cytoplasm of different leucocyte types, including round cells (thin arrows), large cells with granular and lobed nuclei (thick arrows) and thrombocyte-like cells (arrow heads) were Pis1-immunoreactive (brown colour). Leucocytes incubated with latex beads (b, c and d). (b) Blood: some bead-containing phagocytes (thick arrows), round cells (thin arrows) and thrombocyte-like cells (arrow heads) were Pis1-immunoreactive. (c) Head kidney: numerous leucocytes, including phagocytes were Pis1-immunoreactive, phagocytes with intense staining had high bead uptake (thick arrows) than those with a low bead uptake (thin arrows). (d) Spleen: most of the leucocytes including phagocytes (arrows) were Pis1-immunoreactive. (e) Head kidney: numerous vascular tissue (arrows) and some cells in the hematopoietic tissues (*), glomerulus (Gl) and blood vessel (V) were Pis1-immunoreactive; note that glomerulus is surrounded by Bowman’s capsule space (unstained - thin arrow). (f) Trunk kidney: cells in hematopoietic tissue (*), cytoplasm of columnar epithelial cells of collecting tubule (Cb), cells and detached elements in the lumen of renal tubules (arrows) were Pis1-immunoreactive. (g) Spleen: numerous hematopoietic cells were Pis1-immunoreactive (v: blood vessel). Negative control (inset) of the cell samples and serial sections treated with dilution buffer was Pis1-immunonegative (Larger images are seen in Additional file 1: Figure S2).
Ruangsri et al. BMC Veterinary Research 2012 8:46 doi:10.1186/1746-6148-8-46