Immunohistochemistry performed on paraffin sections of healthy Atlantic cod fry. (a) Gill section treated with anti-Pis1 antibody showing immunopositive multi-granular cells (thick arrows) of the gills and a number of chondrocytes (thin arrow) in cartilaginous tissues of gill filament, gill arch (Gac), jaw cartilage (Jcl), and a weak positive reaction for the connective tissue (Ct). (b) Control serial section of (a) treated with Pis1 pre-incubated antibody indicate that all cells and tissues were not immunoreactive. (c) Radial cartilage of anal fin, chondrocytes (arrow) and connective tissue of muscle fibers (*) were Pis1-immunoreactive; (d) control serial section of (c) treated with Pis1 pre-incubated antibody. (e) Gill section treated with anti-cod galectin antibody showing immunoreactive cells scattered throughout the epithelial tissues, while all the chondrocytes were not immunostained.
Ruangsri et al. BMC Veterinary Research 2012 8:46 doi:10.1186/1746-6148-8-46