Table 1

Oligonucleotide primer sequences for amplification of various equine cytokines, SAA and reference genes

Target (gene)

Primer sequence (5'-3')

Product size (bp)

Source/Accesionnumber


SAA

F

CCT GGG CTG CTA AAG TCA TC

169

[25]


R

AGG CCA TGA GGT CTG AAG TG


TNFa

F

GGC CCA GAC ACT CAG ATC AT

73

[9]


R

TTG GGG GTT TGC TAC AAC AT


IL1b3i

F

CAG TCT TCA GTG CTC AGG TTT CTG

84

[9]


R

CAT TGC CGC TGC AGT AAG T


IL-10i

F

GCT GGA GGA CTT TAA GGG TTA C

76

[9]


R

CAT CAC CTC CTC CAG GTA AAA


IL-8i

F

CTT TCT GCA GCT CTG TGT GAA G

189

[9]


R

GCA GAC CTC AGC TCC GTT GAC


β-actin

F

CGT GGG CCG CCC TAG GCA CCA

243

AF035774.1


R

TTG GCC TTA GGG TTC AGG GGG G


β-glucoronidase

F

GCT CAT CTG GAA CTT TGC TGA TTT T

85

XM001493514.2


R

CTG ACG AGT GAA GAT CCC CTT TT


GapdH

F

GGG TGG AGC CAA AAG GGT CAT CAT

418

[26]


R

AGC TTT CTC CAG GCG GCA GGT CAG


IL 6

F

GGA TGC TTC CAA TCT GGG TTC AAT

65

[27]


R

TCC GAA AGA CCA GTG GTG ATT TT


IL-1ra

F

ACA AAT GTG GCT CCT CCA AG

88

NM_001082525


R

TTT CAG AGC GTC AGA AGT GC


Christoffersen et al. BMC Veterinary Research 2012 8:41   doi:10.1186/1746-6148-8-41

Open Data