Table 2

Infection status based specifications of bead-based assay and ELISA tests calculated by ROC analysis

ROC analysis


Maximum Youden Index


test

AUC

SE

P

95% CI

Cut-off

Se

Sp


BBA-TOX

0.911

0.015

< 0.001

0.881-0.940

13.90

0.86

0.96


E1-TOX

0.837

0.018

< 0.001

0.800-0.873

0.369

0.64

0.95


E2-TOX

0.865

0.018

< 0.001

0.829-0.901

0.174

0.76

0.93


E3-TOX

0.930

0.013

< 0.001

0.904-0.956

26.90

0.84

0.99


BBA-TRI

0.855

0.018

< 0.001

0.819-0.890

4.65

0.68

1.00


E-TRI

0.879

0.016

< 0.001

0.849-0.912

0.018

0.72

0.95


ROC analyses of a T. gondii and T. spiralis bead-based assay, three T. gondii and one T. spiralis ELISAs using responses of porcine serum samples from an experimental infection calculated against their infection status. AUC, area under the curve; SE, standard error of AUC; P, P-value; 95% CI, interval at a 95% confidence; Se, sensitivity; Sp, specificity; BBA-TOX, T. gondii antigen coupled bead-based assay; E1-TOX, RIVM in-house T. gondii ELISA, E2-TOX: Safepath T. gondii ELISA, E3-TOX, ID-VET T. gondii ELISA; BBA-TRI, T. spiralis antigen coupled bead-based assay and E-TRI, Safepath T. spiralis ELISA

Bokken et al. BMC Veterinary Research 2012 8:36   doi:10.1186/1746-6148-8-36

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