Cervical epithelial cells of three animals were cultured for 24 h, 48 h and 72 h in different growth medium compositions. Viability and proliferation were measured in triplicates using the Roche WST-1 Proliferation Assay. Extinctions of the media were measured at 450 nm after 30 min incubation with the WST-1 reagent. A) Ham's F-12 relative to the unconditioned medium, and B) conditioned Ham's F-12 with different additives relative to the basic conditioned Ham's F-12.
Miessen et al. BMC Veterinary Research 2012 8:31 doi:10.1186/1746-6148-8-31