Table 5

Amplification of MRI, CH1641 and atypical scrapie strains using enhanced PMCA methods
Strain Genotype Substrate
ARQ VRQ AHQ
MRI scrapie ARQ/ARQ (n = 3) +/+ −/− +/+/+
CH1641 AHQ/AHQ (n = 3) +/+/+ +/+/+ +/+
Atypical scrapie ARQ/ARQ (n = 5) −/−/−/−/− −/−/−/−/− −/−/−/−/−
AHQ/AHQ (n = 2) −/− −/− −/−
ARQ/AHQ (n = 7) −/−/−/−/−/−/− −/−/−/−/−/−/− −/−/−/−/−/−/−

n = number of animals tested. Poly(A) was added to the amplification reactions and the amplifications were carried out for 4 rounds. All analyses were in duplicate except for atypical scrapie samples amplified in ARQ and AHQ substrates which were analysed in single reactions. +/− symbols show the amplification outcome for each animal.

Thorne et al.

Thorne et al. BMC Veterinary Research 2012 8:223   doi:10.1186/1746-6148-8-223

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