Table 5 |
||||
| Amplification of MRI, CH1641 and atypical scrapie strains using enhanced PMCA methods | ||||
| Strain | Genotype | Substrate | ||
| ARQ | VRQ | AHQ | ||
| MRI scrapie | ARQ/ARQ (n = 3) | +/+ | −/− | +/+/+ |
| CH1641 | AHQ/AHQ (n = 3) | +/+/+ | +/+/+ | +/+ |
| Atypical scrapie | ARQ/ARQ (n = 5) | −/−/−/−/− | −/−/−/−/− | −/−/−/−/− |
| AHQ/AHQ (n = 2) | −/− | −/− | −/− | |
| ARQ/AHQ (n = 7) | −/−/−/−/−/−/− | −/−/−/−/−/−/− | −/−/−/−/−/−/− | |
n = number of animals tested. Poly(A) was added to the amplification reactions and the amplifications were carried out for 4 rounds. All analyses were in duplicate except for atypical scrapie samples amplified in ARQ and AHQ substrates which were analysed in single reactions. +/− symbols show the amplification outcome for each animal.
Thorne et al. BMC Veterinary Research 2012 8:223 doi:10.1186/1746-6148-8-223
