|Amplification of MRI, CH1641 and atypical scrapie strains using enhanced PMCA methods|
|MRI scrapie||ARQ/ARQ (n = 3)||+/+||−/−||+/+/+|
|CH1641||AHQ/AHQ (n = 3)||+/+/+||+/+/+||+/+|
|Atypical scrapie||ARQ/ARQ (n = 5)||−/−/−/−/−||−/−/−/−/−||−/−/−/−/−|
|AHQ/AHQ (n = 2)||−/−||−/−||−/−|
|ARQ/AHQ (n = 7)||−/−/−/−/−/−/−||−/−/−/−/−/−/−||−/−/−/−/−/−/−|
n = number of animals tested. Poly(A) was added to the amplification reactions and the amplifications were carried out for 4 rounds. All analyses were in duplicate except for atypical scrapie samples amplified in ARQ and AHQ substrates which were analysed in single reactions. +/− symbols show the amplification outcome for each animal.
Thorne et al. BMC Veterinary Research 2012 8:223 doi:10.1186/1746-6148-8-223