Table 3

Pathological and microbiological findings from five affected calves
Calf no. Pathological changesa E. coli serotypeb Positive samples MLD50for E. coli (CFU)c
1 severe O8 intestine, rectal swabs 5.45 × 109
2 moderate O8 intestine ND d
3 severe O8 intestine, rectal swabs ND
4 severe O8 intestine, rectal swabs ND
5 mild O8 intestine ND

a The pathological changes of intestines from affected calves were estimated by visual examination.

b The serotypes of each strains were determined by agglutination test with 25 prevalent antiserums of pathogenic E. coli.

c MLD50 were determined by intraperitoneally inoculating six mice in each group with 50 μl of serial 10-fold dilutions E. coli and calculated by the method of Hamilton et al. [20].

d ND indicates that the LD50 of these strains was not determined.

He et al.

He et al. BMC Veterinary Research 2012 8:222   doi:10.1186/1746-6148-8-222

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