Figure 1.

Illustration of representative immunohistochemistry in equine normal skin and sarcoid lesions.A) MGMT negative immunoreactivity in a sarcoid sample. Neoplastic fibroblasts are negative for MGMT staining. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. x 240. B) Sarcoid sample showing very weak MGMT immunoreactivity. Some fibroblasts from the neoplastic tissue show a very faint cytoplasmic immunostaining for MGMT. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. IHC. x 240. C) MGMT staining of normal equine skin. Normal fibroblasts from derma displayed nuclear and cytoplasmic staining for MGMT. The different epidermal layers are also stained. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. IHC. x 240. D) Human tonsil tissue section. Epithelial cells show positive MGMT immunostaining. Strepatavidin-biotin peroxidase method. Mayer’s haematoxylin nuclear counterstaining. IHC. x 120.

Altamura et al. BMC Veterinary Research 2012 8:218   doi:10.1186/1746-6148-8-218
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