Table 2 |
||||
| Salmonellavirulence genes detected by PCR analysis | ||||
| Gene | Function | Primer sequence (5′ – 3′) | bp | Reference |
| avrA | Inhibits the proinflammatory, antiapoptotic NF-kappa B pathway | CCTGTATTGTTGAGCGTCTGG | 422 | [8] |
| AGAAGAGCTTCGTTGAATGTCC | ||||
| ssaQ | Secretion system apparatus protein, component of second T3SS | AATGAGCTGGGTAGGGTGTG | 216 | This study |
| ATGCAACGCTAGCTGATGTG | ||||
| mgtC | Intramacrophage survival protein | TGACTATCAATGCTCCAGTGAAT | 677 | [8] |
| ATTTACTGGCCGCTATGCTGTTG | ||||
| siiD | HLYD family secretion protein | GTTCATGGTCAGGGCGTTAT | 416 | This study |
| GCAAGCAATGCGAGTTCTTT | ||||
| sopB | Translocated effector protein (phosphoinositide phosphatase) via T3SS | TAACGTCAATGGCAAACCAA | 334 | This study |
| CCCTCATAAGCACTGGGAAA | ||||
| gipA | Peyer’s patch-specific virulence factor | GCAAGCTGTACATGGCAAAG | 212 | [9] |
| GGTATCGGTGACGAACAAAT | ||||
| gogB | Type III-secreted substrate of the infection process | GCTCATCATGTTACCTCTAT | 598 | [10] |
| AGGTTGGTATTTCCCATGCA | ||||
| sopE | Translocated T3SS effector protein | CGAGTAAAGACCCCGCATAC | 363 | [10] |
| GAGTCGGCATAGCACACTCA | ||||
| gtgB | Translocated T3SS effector protein | TGCACGGGGAAAACTACTTC | 436 | [9] |
| TGATGGGCTGAAACATCAAA | ||||
| sspH1 | Salmonella secreted protein H1 | TGCAGAAAAAGGGGAATACG | 246 | This study |
| GCAGCCTGAAGGTCTGAAAC | ||||
| sspH2 | Salmonella secreted protein H2 | GCACAACTGGCTGAAGATGA | 203 | This study |
| TTTCCCAGACGGAACATCTC | ||||
| gtgE | SPI2 type III secreted effector protein | AGGAGGAGTGTAAAGGT | 1114 | [11] |
| GTAGAACTGGTTTATGAC | ||||
| sodC1 | Periplasmmic Cu, Zn-superoxide dismutases | TATTGTCGCTGGTAGCTG | 468 | [11] |
| CAGGTTTATCGGAGTAAT | ||||
| spvC | Spv region promotes rapid growth and survival within the host | ACTCCTTGCACAACCAAATGCGGA | 571 | [12] |
| TGTCTTCTGCATTTCGCCACCATCA | ||||
| invA | Enables the bacteria to invade cells | ACAGTGCTCGTTTACGACCTGAAT | 244 | [12] |
| AGACGACTGGTACTGATCGATAAT | ||||
| stfE | Minor fimbrial subunit of the Salmonella Typhi flagella | ATTTGGCAATGTGTTGACGA | 185 | This study |
| TTTGCAGACGGATACCCAAT | ||||
| safC | Pilin outer membrane usher protein | CTCGCTGTCATTGAACTGGA | 158 | This study |
| CACCGTGTGATGGTGAAGTC | ||||
| csgA | Major fimbrial subunit of thin curled fimbriae | GGATTCCACGTTGAGCATTT | 212 | This study |
| CGGAGTTTTTAGCGTTCCAC | ||||
| ipfD | The Ipf fimbrial operon mediates adhesion to Peyer’s patches | TTCCCTCAATACGCAGGAAG | 183 | This study |
| CTCAGGGCTGTGAACTCTCC | ||||
| bcfC | Bovine colonization factor, fimbrial usher | CAGCTTTTCATGACGCGATA | 241 | This study |
| CAATGTCTCTGGTTGCGAGA | ||||
| stbD | Stability protein involved in a toxin-antitoxin system and in plasmid stability | GGCTGTAATATTCGCCGGTA | 201 | This study |
| GCACGCCCTATTCCAGTAAA | ||||
| pefA | Major fimbrial subunit of the plasmid encoded fimbria | ACACGCTGCCAATGAAGTGA | 450 | [18] |
| ACTGCGAAAGATGCCACAGA | ||||
| fimA | Type 1 major fimbrial unit | CCTTTCTCCATCGTCCTGAA | 85 | This study |
| TGGTGTTATCTGCCTGACCA | ||||
| agfA | Aggregative fimbria A | GGATTCCACGTTGAGCATTT | 312 | [18] |
| GTTGTTGCCAAAACCAACCT | ||||
Borriello et al. BMC Veterinary Research 2012 8:201 doi:10.1186/1746-6148-8-201
