Figure 13.

Ghrelin promotes migration of canine mammary carcinoma cells. (A) Quantification of migration of examined cell lines (CMT-W1, CMT-W1M, CMT-W2, CMT-W2M) represented as relative florescent units obtained by florescent plate reader Infinite 200 PRO Tecan™ (Ex. 485, Em. 530). Ghrelin treatment for 24 hrs at the concentration of 1 nM and 10 nM increased cancer cells migration through membrane of Boyden chamber assay in CMT-W1M, CMT-W2, CMT-W2M cell lines. In CMT-W1 cell line only grelin concentration of 10 nM was effective. The experiment has been conducted three times. The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). One-way ANOVA followed by Tukey HSD post-hoc test and unpaired t-test were applied. p < 0.05 was marked as *, p < 0.01 was marked as **. (B) Fluorescence microscopic analysis of the migrated CMT-W2 cells using Olympus microscopy BX60 at x4 magnification.