Table 1

Comparison of BLV proviral detection by BLV-CoCoMo-qPCR, the TaqMan MGB assay, and TaKaRa cycleave PCR
pBLV-IF CoCoMo-qPCRa Lew Methodb TaKaRa
(copy number) Cycleave PCRc
100 3/3d 3/3 3/3
50 3/3 3/3 3/3
25 3/3 3/3 3/3
12.5 3/3 3/3 3/3
6.25 3/3 3/3 3/3
3.125 3/3 3/3 3/3
1.5625 3/3 0/3 3/3
0.78125 3/3 0/3 2/3
0 0/3 0/3 0/3

a BLV LTR genes were detected by BLV-CoCoMo-qPCR (Jimba et al., 2010).

b BLV pol gene was detected by TaqMan MGB assay developed by Lew et al. (Lew et al., 2004).

c BLV tax gene was detected by the cycleave PCR BLV detection kit (TaKaRa Bio inc.).

d Number detected per number tested.

Jimba et al.

Jimba et al. BMC Veterinary Research 2012 8:167   doi:10.1186/1746-6148-8-167

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