Table 2

Results of fragment analyses and sequencing of PCR products obtained from samples of commercial dairy farms
Locus Fragment PCR product length (bp) Number of tandem repeats
Fragment analysis Sequencing Fragment analysis1 Sequencing
Ms27 1 285 282 2.4 2.0
2 290 288 3.3 3.0
3 295 294 4.2 4.0
Ms28 1 189 190 2.8 3.0
2 201 202 4.8 5.0
3 207 208 5.8 6.0
4 213 214 6.8 7.0
Ms34 1 107 112 1.2 2.0
2 113 118 2.2 3.0
3 124 130 4.1 5.0
4 136 142 6.1 7.0
5 143 148 7.1 8.0
6 148 153 8.1 9.0
Ms20b 1 252 258 4.4 4.5
2 269 273 4.9 5.0
3 349 354 7.3 7.5
Ms24 1 165 163 9.3 9.0
2 179 177 11.3 11.0
3 192 191 13.1 13.0
4 287 289 26.7 27.0
Ms31 1 130 136 2.1 3.0
2 145 150 4.3 5.0

1The numbers of tandem repeats from fragment sizes were calculated by dividing the sizes of the measured PCR products minus primer binding sites and flanking regions by the corresponding repeat length.

de Bruin et al.

de Bruin et al. BMC Veterinary Research 2012 8:165   doi:10.1186/1746-6148-8-165

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