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Open Access Highly Accessed Research article

Identification of the NLS and NES motifs of VP2 from chicken anemia virus and the interaction of VP2 with mini-chromosome maintenance protein 3

Jai-Hong Cheng1, Shyang-Chwen Sheu2, Yi-Yang Lien3, Meng-Shiunn Lee4, His-Jien Chen5, Wen-Hong Su1 and Meng-Shiou Lee6*

Author Affiliations

1 Department of Medical Research, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan

2 Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan

3 Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan

4 Department of Medical Research, Tung's Taichung Metro Harbor Hospital, Taichung, Taiwan

5 Department of Safety, Health and Environmental Engineering, Mingchi University of Technology, Taipei, Taiwan

6 School of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung, Taiwan

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BMC Veterinary Research 2012, 8:15  doi:10.1186/1746-6148-8-15

Published: 7 February 2012

Additional files

Additional file 1:

The effect of LMB treatments on the various mutants of VP2. The truncated mutants of VP2 in Figure 3A were all treated with LMB (+) (20 ng/ml) and LMB (-) (PBS buffer) for 1 h at 37°C. VP3-GFP is LMB sensitive and was used as a positive control. The distribution of GFP was monitored by fluorescence microscopy.

Format: TIFF Size: 3.1MB Download file

Open Data

Additional file 2:

Identification of protein-protein interactions with VP2 surveyed by co-immunoprecipitation. At 48 h post-transfection with plasmids encoding GFP (as the Control) or Flag-VP2-GFP (WT), cell lysates were immunoprecipitated by Flag M2 beads and immunoblotted against VP2, MCM3, CDC7, SMC2, PCNA, and H2B antibodies. The nuclear extracts containing VP2-GFP are designated as N.

Format: TIFF Size: 3.1MB Download file

Open Data