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Open Access Highly Accessed Research article

Unexpected genetic diversity of Mycoplasma agalactiae caprine isolates from an endemic geographically restricted area of Spain

Christian De la Fe1*, Joaquín Amores1, Florence Tardy2, Eveline Sagne34, Laurent-Xavier Nouvel34 and Christine Citti34

Author Affiliations

1 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo s/n, 30100, Murcia, Spain

2 UMR Mycoplasmoses des Ruminants, Anses, Laboratoire de Lyon, 31 Avenue Tony Garnier, 69364, Lyon Cedex 07, France

3 INRA, UMR 1225, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076, Toulouse Cedex 3, France

4 Université de Toulouse, INP-ENVT, UMR 1225, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076, Toulouse Cedex 3, France

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BMC Veterinary Research 2012, 8:146  doi:10.1186/1746-6148-8-146

Published: 27 August 2012

Additional files

Additional file 1:

Table S1. VNTR nucleotide sequences.

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Additional file 2:

Table S2. Profiles obtained with the probe A3F, the set of individual Vpma probes and the AGA-BOV probes used in this study.

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Additional file 3:

Table S3. Description of additional PCRs conducted in this study, the results of which are summarized in Table 1.

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Additional file 4:

Figure S1. Illustration of some genomic differences between M. agalactiae strains PG2 and 5632 that were used to design new PCR typing assays.

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Additional file 5:

Figure S2. Geographic origin of Mycoplasma agalactiae isolates.

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Additional file 6:

Table S4. Primer sequences.

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