E2-induced SBD-1 expression in ovine oviduct epithelial cells requires GPR30 and ERs. (A) The cells were treated with the GPR30 agonist G1 (10-7 M) and E2 (10-8 M). (B) Other groups of cells were incubated with the inhibitor ICI 182,780 (Anti-ER 10-7 M, 1 h) and then added with E2 (10-8 M). Furthermore, a blank control (water substituted for cDNA) was included in each qPCR reaction mixture, and a control was set up containing water instead of E2 or/and G1/ ICI 182,178 in every group. (C, D) In the magnified inset, there were statistically significant changes between the two different treatments at different times from A or B. Total RNA extracted at the indicated times from the ovine oviduct epithelial cells (n = 3). Values are given as mean ± standard deviation. (*: p < 0.05 vs control, **: p < 0.01 vs control).
Wen et al. BMC Veterinary Research 2012 8:143 doi:10.1186/1746-6148-8-143