Table 5

Detection limit of virus particles with rRT-PCR assays and cRT-PCR assaysa
ERV Strainsb TCF Titer Assay name
rRT-PCR Assays cRT-PCR Assays
ERAV ERBV ERAV ERBV
ERAV ERBV1 ERBV2 ERAV 5'-UTR ERAV POLY1 ERAV POLY2 ERBV 5'-UTR ERBV OUTER 1d ERBV OUTER 2d ERBV INNER 1d ERBV INNER 2d ERBV Poly 1 ERBV Poly 2
ERAV 10−7 10−6 NAc NA 10−6 10−5 10−5 NA NA NA NA NA NA NA
ERBV 10−7 NA 10−7 10−4 NA NA NA 10−1 10−5 10−4 10−4 10−4 10−4 10−5

a Serial decimal dilutions of ERAV and ERBV were tested in a comparison study by virus isolation in cell culture, rRT-PCR and standard RT-PCR assays. Numbers shown on the table represent the serial virus dilution.

b ERAV and ERBV prototype strains were obtained from NVSL.

c Not applicable.

d The primers used in these four assays were obtained from a nested RT-PCR developed by Black et al. (2007).

Lu et al.

Lu et al. BMC Veterinary Research 2012 8:120   doi:10.1186/1746-6148-8-120

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