|Detection limit of virus particles with rRT-PCR assays and cRT-PCR assaysa|
|ERV Strainsb||TCF Titer||Assay name|
|rRT-PCR Assays||cRT-PCR Assays|
|ERAV||ERBV1||ERBV2||ERAV 5'-UTR||ERAV POLY1||ERAV POLY2||ERBV 5'-UTR||ERBV OUTER 1d||ERBV OUTER 2d||ERBV INNER 1d||ERBV INNER 2d||ERBV Poly 1||ERBV Poly 2|
a Serial decimal dilutions of ERAV and ERBV were tested in a comparison study by virus isolation in cell culture, rRT-PCR and standard RT-PCR assays. Numbers shown on the table represent the serial virus dilution.
b ERAV and ERBV prototype strains were obtained from NVSL.
c Not applicable.
d The primers used in these four assays were obtained from a nested RT-PCR developed by Black et al. (2007).
Lu et al.
Lu et al. BMC Veterinary Research 2012 8:120 doi:10.1186/1746-6148-8-120