Table 1

Literature data regarding the diagnostic specificity and sensitivity of ELISA tests used to detect antibodies againstM. agalactiae
Kit origin Antigen type Diagnostic specificity Diagnostic sensitivity Tested on References
Anses Sophia Antipolis France (a) Total antigen 12 strains mixture 94% 48% 1017 sheep sera in 52 flocks from the French Pyrénées-Atlantiques department [8]
Intervet(CHEKit®)(a) Total antigen PG2 reference strain 99% 72% 1017 sheep sera in 52 flocks from the French Pyrénées-Atlantiques department [8]
99% 76% 30 goat sera collected from an infected herd and 97 uninfected sheep sera from New Zealand [7]
76% 74% 223 CA infected and 120 CA free sheep from Italy [6]
POURQUIER-ELISAM. agalactiae (b) Fusion protein P48 99% 82% 1017 sheep sera in 52 flocks from the French Pyrénées-Atlantiques department [8]
100% 56% 30 goat sera collected from an infected herd and 97 uninfected sheep sera from New Zealand [7]
- 57% 223 CA infected and 120 CA free sheep from Italy [6]
Italy (c) Fusion proteins P80 and P55 97% 94% 223 CA infected and 120 CA free sheep from Italy [6]
Brazil (c) Total antigen Unknown strain 95% 7789% 86 sera from 44 bacteriologically positive and 42 bacteriologically negative Brazilian goats [5]

(a) Formerly used commercial kit, (b) Test currently marketed and included in the study.

(c) Non commercial tests.

CA: contagious agalactia.

Poumarat et al.

Poumarat et al. BMC Veterinary Research 2012 8:109   doi:10.1186/1746-6148-8-109

Open Data