Kinetics of serological responses in goats inoculated experimentally with three strains ofM. agalactiae. (A) Examples of immunoblotting obtained after inoculation: a) with PG2 reference strain; b) with 5632 strain; c) with14628 strain. Lane: 1 = J0, 2 = J + 6, 3 = J + 9, 4 = J + 12, 5 = J + 16, 6 = J + 19, 7 = J + 22, 8 = J + 30, days after first inoculation. Lane T: positive reference serum PAL97. Total proteins from the of M. agalactiae reference strain PG2 (clone 55–5)  were used as antigen. (B and C) Distribution of serum titers obtained respectively with TA-ELISA and with P48-ELISA. PG2: green curve, 5632: red curve, 14628: blue curve. Each curve corresponds to the average response of two animals for strains PG2 and 5632, three animals for strain 14628. Each point is an average of four repeated measures per animal. The manufacturer’s suggested cut-off point is represented by the purple broken line. * Days of inoculation are symbolized with a star. J + x: number of days after the initial inoculation.
Poumarat et al. BMC Veterinary Research 2012 8:109 doi:10.1186/1746-6148-8-109