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Open Access Methodology article

Serological testing of cattle experimentally infected with Mycoplasma mycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns

Evelyn Schubert12, Konrad Sachse2, Jörg Jores3 and Martin Heller14*

Author Affiliations

1 National Reference Laboratory for CBPP, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Naumburger Str. 96a, 07743 Jena, Germany

2 Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Naumburger Str. 96a, 07743 Jena, Germany

3 International Livestock Research Institute (ILRI), Old Naivasha Road, P.O. Box 30709, 00100 Nairobi, Kenya

4 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Naumburger Str. 96a, 07743 Jena, Germany

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BMC Veterinary Research 2011, 7:72  doi:10.1186/1746-6148-7-72

Published: 18 November 2011

Additional files

Additional file 1:

Results of in-house CFT vs. CIRAD CFT from sera of Panel 2. Humoral immune response of animals from Panel 2 (Short-term Afadé trial) as characterised by in-house CFT and CIRAD CFT. End-point titres of both CFTs are shown at different time points of infection.

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Additional file 2:

Examination of Panel 2 sera using cELISA. Humoral immune response of animals from Panel 2 (Short-term Afadé trial) characterised by cELISA. The percentage inhibition value (INH%) for each serum sample was calculated using the formula: INH% = (ODmab - ODsample)/(ODmab - ODconjugate) × 100%, ODmab = Control only with monoclonal antibody and without serum (0% inhibition), ODsample = OD of the serum sample, ODconjugate = Control without monoclonal antibody and serum (100% inhibition). The cut-off for positive samples was set at INH% of 50%. Sera with an inhibition value between 40% and 50% were considered doubtful. All sera were examined in duplicate. ND = not done.

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Additional file 3:

Examination of Panel 3 sera using in-house CFT. Examination of the 7 sera from the long-term Afadé trial (Panel 3) using in-house CFT. End-point titres of the CFT were shown until 34 weeks p.i.

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Additional file 4:

Examination of Panel 3 sera using CIRAD CFT. Examination of the 7 sera from the long-term Afadé trial (Panel 3) using CIRAD CFT. End-point titres of the CFT were shown until 34 weeks p.i.

Format: TIFF Size: 67KB Download file

Open Data

Additional file 5:

Examination of Panel 3 sera using cELISA. Examination of the 7 sera from the long-term Afadé trial (Panel 3) using cELISA. Data of the cELISA were given in percentage inhibition and shown until 34 weeks p.i.

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Open Data

Additional file 6:

Proposal for modification of the current OIE protocol for IBT. To improve the reproducibility of IBT results, the authors of the present paper recommend two modifications to the protocol of the OIE Manual.

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