Table 5

Effect of weaning induced stress on the relative gene expression of toll-like receptor (TLR) 4, glucocorticoid receptor (GR)α, nuclear factor kappa B (NFκB), Fas, p21, CD62l, haptoglobin and bactericidal/permeability increasing protein (BPI) in weaned beef calves.

Gender

Location

s.e.

Time

s.e.

P-Values




Variable

Male

Female

Near

Away

0

1

3

7

G

L

T

I




TLR4

9.4

7.5

8.0

8.9

1.4

5.2

8.3b

8.9c

11.5c

1.3

NS

NS

***

NS

GRα

13.4

16.5

16.4

13.5

2.1

6.4

19.8c

16.9c

16.6c

2.5

NS

NS

***

NS

NFκB1

2.9

2.9

2.8

3.0

0.27

2.5

3.1b

2.9

3.1b

0.25

NS

NS

*

NS

NFκB2

2.9

2.8

2.9

2.7

0.22

2.8

2.9

2.7

2.8

0.24

NS

NS

NS

NS

Fas

27.9

24.5

25.7

26.8

4.8

9.7

33.6c

26.4c

35.2c

5.1

NS

NS

***

NS

p21

5.8

5.5

5.4

5.9

0.70

6.2

4.9

5.7

5.6

0.71

NS

NS

NS

NS

CD62L

33.7

35.5

34.4

34.8

5.1

26.0

34.8b

37.5c

40.1c

4.3

NS

NS

***

NS

Haptoglobin

14.4

16.6

13.7

17.3

3.9

12.8

16.3

17.7

15.2

3.5

NS

NS

NS

NS

BPI

14.8

15.6

16.5

13.9

2.5

18.3

16.7

14.8a

11.1c

2.1

NS

NS

***

NS


The values are expressed as least squares means (Lsmeans) and s.e.

G = gender, L = location, T = time, I = interaction, NS = not significant (P > 0.05). * = P < 0.05, ** = P < 0.01, *** = P < 0.001.

a, b, cWithin rows, Lsmeans differ from pre-weaning baseline by P < 0.05, P < 0.01 and P < 0.001, respectively.

O'Loughlin et al. BMC Veterinary Research 2011 7:45   doi:10.1186/1746-6148-7-45

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