Additional file 3.

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CP1503 (suspect-BSE negative). Steer, examined prior to cull at 87 months after intracerebral inoculation with a pool of spleens collected from cattle orally exposed to BSE affected brainstem and killed 18 mpi (study 2). This steer is slightly over-reactive to cranial nerve testing. It also over-reacts to touch on the neck (recorded as "nervous on neck prick"). It does not over-react to sudden flash light but a head tremor is evident during the procedure. The steer displays marked hind limb hypermetria, which is most pronounced as it reaches the end of the corridor. The steer also appears apprehensive in the corridor, as indicated by hesitation in passing the examiner and a very alert facial expression with ear movements when close to the camera. Note also the head and neck tremor when the animal stands near the camera at the end of the clip. This steer was negative for BSE by postmortem tests. Histopathologically, there was no evidence of a cerebellar lesion which may have produced hypermetria and head tremor.

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Konold et al. BMC Veterinary Research 2010 6:53   doi:10.1186/1746-6148-6-53