Figure 4.

Western immunoblot of caudal medulla of the atypical scrapie cases. WB using monoclonal antibody Sha31, showing the consistency of blot characteristics between field case, experimental and sub-passaged atypical scrapie. Lanes 1 and 10 Biotinylated markers. Lane 2 Case 4. Lane 3 Case 8 (the sub-passage of Case 4). Lanes 4 and 5 Cases 7 and 5, both sub-passages of Case 1 (not shown). Lane 6 Field case atypical scrapie positive control. Lane 7 Field case classical scrapie control. Lane 8 Bovine BSE positive control. Lane 9 Scrapie negative control.