Figure 4.

P-type determination showing a selection of human and bovine RV samples. (Amplicon sizes are shown in parenthesis). A 1.5% agarose gel was used to visualize P-typing amplicons from a semi-nested multiplex PCR reaction. Lane 1: 100 bp Marker (New England Biolabs), Lane 2: P[4] (HRV-1 – 345 bp), Lane 3: P[8] (HRV-2 – 484 bp), Lane 4: P[5] (Bovine RV-1 – 555 bp), Lane 5: P[11] (Bovine RV-2 – 315 bp), Lane 6: P[11] (GirRV-315 bp), Lane 7: P [5+11] (Bovine RV-3 – 555, 315 bp), Lane 8: P[1] (Bovine RV-4 – 662 bp).