|
Average OD readings for serum dilution and protein G combination. |
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| Serum dilution |
Protein G dilution |
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| 1/10 |
1/100 |
1/200 |
1/500 |
1/1000 |
1/2500 |
2.5 μg/ml |
1 μg/ml |
0.5 μg/ml |
0.05 μg/ml |
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|
|
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| WB1 + WB4 |
S2/S1 |
9,2805 |
9,2610 |
9,6143 |
9,5895 |
9,1172 |
6,4596 |
6,3067 |
8,3368 |
10,702 |
11,129 |
| S3/S1 |
10,465 |
11,061 |
11,38 |
11,574 |
11,730 |
9,1666 |
7,5116 |
10,252 |
12,074 |
13,751 |
|
| S1 |
0,1758 |
0,1089 |
0,0855 |
0,0596 |
0,0465 |
0,0355 |
0,1428 |
0,0981 |
0,0728 |
0,0275 |
|
| S2 |
1,0053 |
0,8198 |
0,7176 |
0,57 |
0,4293 |
0,2521 |
0,7832 |
0,729 |
0,6658 |
0,3514 |
|
| S3 |
1,0706 |
0,9049 |
0,7892 |
0,6605 |
0,5357 |
0,3438 |
0,8763 |
0,8273 |
0,7576 |
0,4086 |
|
| WB2 |
S2/S1 |
1,4873 |
1,2624 |
1,2840 |
1,1513 |
1,2033 |
1,1837 |
1,2756 |
1,2966 |
1,3386 |
1,1372 |
| S3/S1 |
5,9384 |
5,5608 |
5,0313 |
3,6979 |
2,8577 |
2,3318 |
4,0252 |
4,7892 |
5,1064 |
3,0244 |
|
| S1 |
0,0941 |
0,0669 |
0,055 |
0,0523 |
0,0414 |
0,0305 |
0,094 |
0,062 |
0,0491 |
0,0217 |
|
| S2 |
0,1473 |
0,0841 |
0,0719 |
0,061 |
0,0504 |
0,0353 |
0,125 |
0,0844 |
0,0657 |
0,0248 |
|
| S3 |
0,5771 |
0,3818 |
0,2885 |
0,2023 |
0,1224 |
0,0686 |
0,4152 |
0,3324 |
0,2779 |
0,0683 |
|
| WB2 |
S2/S1 |
3,1229 |
2,4333 |
2,6135 |
2,0944 |
1,8366 |
1,4173 |
2,2195 |
2,3216 |
2,5731 |
1,8978 |
| S3/S1 |
4,0662 |
3,3376 |
4,0625 |
3,1850 |
2,8014 |
2,1894 |
3,0310 |
3,3525 |
3,7237 |
2,9875 |
|
| S1 |
0,1119 |
0,0671 |
0,054 |
0,0611 |
0,0485 |
0,0383 |
0,1052 |
0,0704 |
0,0543 |
0,0241 |
|
| S2 |
0,3505 |
0,1645 |
0,145 |
0,1318 |
0,0914 |
0,0543 |
0,2504 |
0,8477 |
0,1515 |
0,0464 |
|
| S3 |
0,41 |
0,2215 |
0,2269 |
0,1994 |
0,1374 |
0,0839 |
0,3213 |
0,8577 |
0,213 |
0,0731 |
|
|
All protein G concentrations at each serum dilution, and all serum dilutions at each protein G concentration. WB1+WB4: Sum of wild boar 1 and wild boar 4 results. S2/S1 and S3/S1: Increase rate between pre- and post-immunization results. S1, S2 and S3: OD values. | |||||||||||
Aurtenetxe et al. BMC Veterinary Research 2008 4:43 doi:10.1186/1746-6148-4-43 |
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