Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens

doi:10.1186/1746-6148-4-37

BMC Veterinary Research

Volume 4

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Pardo BG
Fernández C
Millán A
Bouza C
Vázquez-López A
Vera M
Alvarez-Dios JA
Calaza M
Gómez-Tato A
Vázquez M
Cabaleiro S
Magariños B
Lemos ML
Leiro JM
Martínez P

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Pardo BG
Fernández C
Millán A
Bouza C
Vázquez-López A
Vera M
Alvarez-Dios JA
Calaza M
Gómez-Tato A
Vázquez M
Cabaleiro S
Magariños B
Lemos ML
Leiro JM
Martínez P
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Research article

Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens

Belén G Pardo¹ , Carlos Fernández¹ , Adrián Millán¹ , Carmen Bouza¹ , Araceli Vázquez-López¹ , Manuel Vera¹ , José A Alvarez-Dios² , Manuel Calaza² , Antonio Gómez-Tato³ , María Vázquez⁴ , Santiago Cabaleiro⁴ , Beatriz Magariños⁵ , Manuel L Lemos⁵ , José M Leiro⁵ and Paulino Martínez¹

¹Departamento de Genética. Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus de Lugo, 27002 Lugo, Spain

²Departamento de Matemática Aplicada, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain

³Departamento de Geometría y Topología, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain

⁴Cluster de la Acuicultura de Galicia (CETGA), Punta de Couso s/n, 15965, Aguiño (Ribeira), A Coruña, Spain

⁵Departamento de Microbiología y Parasitología. Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain

author email corresponding author email

BMC Veterinary Research 2008, 4:37doi:10.1186/1746-6148-4-37


Published:	25 September 2008

Additional files

Additional file 1:

Comparison of gene expression profiles regulated in response to turbot pathogens. The total number and distribution among libraries (Aeromonas, Philasterides, control) of sequences from contigs with 6 or more sequences is presented. The homology with public databases (e-value), the function and the probability of departure from the null hypothesis of even distribution of sequences among libraries is shown for each contig.

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Additional file 2:

Comparison of ESTs grouped according to GO terms among the three turbot cDNA libraries. contigs of annotated genes are shown grouped according to GO term categories (Molecular function; Biological process; Cellular component) and split into the three turbot libraries from which sequences were obtained. The expression pattern of each gene at each library is evaluated as the amount of sequences found. This pattern is compared among libraries and examined for its deviation from an even distribution among libraries by a chi-square test.

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