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Open Access Research article

Molecular characterization of Anaplasma platys strains from dogs in Sicily, Italy

José de la Fuente12*, Alessandra Torina3, Victoria Naranjo2, Silviane Nicosia3, Angelina Alongi3, Francesco La Mantia3 and Katherine M Kocan1

Author Affiliations

1 Department of Veterinary Pathobiology, College of Veterinary Medicine, 250 McElroy Hall, Oklahoma State University, Stillwater, OK 74078, USA

2 Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain

3 Istituto Zooprofilattico Sperimentale della Sicilia, Via G. Marinuzzi n°3, 90129 Palermo, Italy

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BMC Veterinary Research 2006, 2:24  doi:10.1186/1746-6148-2-24

Published: 26 July 2006

Abstract

Background

The genetic diversity of Anaplasma platys (Rickettsiales: Anaplasmataceae) strains is currently poorly defined. The present study was designed to characterize A. patys strains in dogs from Palermo, Sicily, Italy, using a combination of PCR and sequence analysis of the 16S rDNA, heat shock operon groESL and citrate synthase (gltA) genes.

Results

Blood was collected from 344 dogs (111 pet dogs, 122 pound dogs and 111 hunting dogs) during 2003–2005 in the Province of Palermo, Sicily, Italy. The prevalence of A. platys in dogs in Sicily, as demonstrated by PCR and sequence analysis of the 16S rDNA, groESL and gltA genes, was 4%. None of the samples were positive for A. marginale, A. centrale, A. ovis and A. phagocytophilum DNA. Three different gltA genotypes of A. platys were identified in dogs from Sicily. Two of the gltA sequences of Sicilian A. platys strains were different from sequences reported previously. However, one of the gltA, 16S rDNA and groESL sequences were identical to the sequence of A. platys strains from other regions of the world characterized previously.

Conclusion

At least three different strains of A. platys were identified in dogs from Sicily by PCR and sequence analyses of the 16S rDNA, groESL and gltA genes. The results reported herein suggested that genetic diversity of A. platys strains may be similar to A. ovis, but lower than the diversity reported for A. marginale and A. phagocytophilum. This lower genetic diversity may have resulted from restricted movement of infected hosts compared to A. marginale-infected cattle and/or the limited host range of A. ovis and A. platys as compared with A. phagocytophilum. These results expand our knowledge about A. platys and encourage further research for analysis of the genetic variation of A. platys strains worldwide.