Table 1

Measurement of scrapie infectivity in fish tissues by mouse bioassay. Brains from recipient mice inoculated with turbot or trout tissues from either oral or multiple parenteral routes were assayed for PrPSc by Western blot. Whenever samples were available, histology and/or immunohistochemistry was performed. Asterisks indicate mice contributing to the lesion profiles curve of the fish-passaged 139A (Figure 3)

A. Tissues from infected fish via the oral route


Turbot

Trout



Days after infection

Muscle

Intestine

Brain

Spleen

Muscle

Intestine

Brain

Spleen


1

0/10

0/8

0/8

-

0/10

1/8

0/5

-

15

0/7

0/8

0/6

-

0/10

0/8

0/13

-

30

0/10

0/8

0/10

-

0/8

0/8

0/9

-

60

0/9

0/6

0/8

-

0/7

0/6

0/6

-

90

0/7

0/8

0/9

-

0/10

0/11

0/10

-


B. Tissues from infected fish via multiple parenteral routes


15

-

-

2/8

4/7*

-

-

0/9

1/9*

90

-

-

1/7*

0/8

-

-

0/11

0/11


Ingrosso et al. BMC Veterinary Research 2006 2:21   doi:10.1186/1746-6148-2-21

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