Histopathological changes in nuchal ligament. A. Only thin septa separate bundles of collagen and elastic fibers in a normal nuchal ligament. Hematoxylin & eosin, magnification × 200 (× 200). B. In DSLD – affected tissue streaks of proteoglycans (▶) separate bundles of collagen and elastic fibers. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains very lightly normal nuchal ligament, magnification × 200. D. PGs accumulated among bundles stain intensively with alcian blue (▶) in DSLD-affected nuchal ligament.
Halper et al. BMC Veterinary Research 2006 2:12 doi:10.1186/1746-6148-2-12