Open Access Research article

Highland cattle and Radix labiata, the hosts of Fascioloides magna

Roman Leontovyč1, Monika Košťáková1, Veronika Siegelová1, Klára Melounová1, Jan Pankrác1, Kristýna Vrbová1, Petr Horák1 and Martin Kašný12*

Author Affiliations

1 Faculty of Science, Department of Parasitology, Charles University in Prague, Viničná 7, Prague 128 44, Czech Republic

2 Faculty of Science, Department of Botany and Zoology, Masaryk University, Kotlářská 2, Brno 611 37, Czech Republic

For all author emails, please log on.

BMC Veterinary Research 2014, 10:41  doi:10.1186/1746-6148-10-41

Published: 11 February 2014

Abstract

Background

Fascioloides magna is a pathogenic fluke introduced to Europe ca 140 years ago. As it is spreading over the continent, new intermediate and definitive hosts might be involved in transmission of the parasite. In Europe, several studies reported potential new intermediate snail hosts (Radix spp.) for F. magna, and also several cases of fascioloidosis of wild and domestic animals were published. However, the data based on molecular and histological analyses confirming these findings remained unreported. This study aims to refer to unique findings of F. magna in European snails and domestic animals (the first observation in the Czech Republic in the last 30 years) and demonstrate the use of molecular techniques in determination of F. magna.

Results

Two snails of R. labiata naturally infected with F. magna were found; mature cercariae and daughter rediae were observed. Maturity of cercariae was checked by histological methods, however, their ability to encyst was not confirmed. Co-infection of F. magna and Fasciola hepatica in the liver of two highland cattle bulls was proved. Adult fasciolid flukes producing eggs were found in the liver pseudocysts (F. magna) and the bile ducts (F. hepatica). Identification of intermediate hosts, intramolluscan stages, adult flukes and eggs was performed by sequencing the ITS2 region. Connection of F. magna pseudocysts with the gut (via the bile ducts) was not confirmed by means of histological and coprological examinations.

Conclusions

For the first time, Radix labiata was confirmed as the snail host for F. magna under natural conditions and, together with the finding of F. magna infection in cattle, we can expect further transmission of F. magna from wildlife to livestock in localities shared by these hosts.

Keywords:
Fascioloides magna; Fasciola hepatica; Radix labiata; Galba truncatula; Highland cattle; Molecular determination; ITS2; Histology; Pseudocyst