Comparison of the Improved SEPT9 PCR Detection Protocol with the PRESEPT Method using Analytical Specimens. Methylated SEPT9 DNA was measured in pooled normal human plasma spiked with various concentrations of wholly methylated human DNA. Multiple samples of each DNA concentration were prepared and pooled, allowing for the direct comparison of the PRESEPT and improved PCR detection methods using identical DNA substrates. PCR, polymerase chain reaction.
Warren et al. BMC Medicine 2011 9:133 doi:10.1186/1741-7015-9-133