miR-124 and miR-137 are down-regulated in anaplastic astrocytomas and glioblastoma multiformes and are up-regulated in glioblastoma multiforme cell lines following treatment with DNA demethylating agents. (A) Expression of high-grade astrocytomas-microRNAs in individual tumor samples measured relative to let-7a (black dots) and miR-16 (white dots). Sample classes are glioses, anaplastic astrocytomas and glioblastoma multiformes. (B) Glioblastoma multiforme cell lines (U87 and U251) were treated with 5-aza-dC at 1 μM (Aza.1) or 5 μM (Aza.5) alone, trichostatin A (100 ng/ml) alone, or combinations of both agents. MicroRNA expression was measured relative to let-7a and normalized to vehicle control (dimethyl sulfoxide). Error bars represent standard deviation of triplicate polymerase chain reactions from a single experimental set. Similar results were obtained in independent experiments (see Additional file 7).
Silber et al. BMC Medicine 2008 6:14 doi:10.1186/1741-7015-6-14