MitoQ inhibits caspase-1 activation through suppression of TXNIP binding to NLRP3 during colitis. (A) Western blotting analysis reveals expression of the NLRP3 inflammasome components in mouse colon homogenates. (B) Dissociation of the TXNIP-TRX complex upon MitoQ treatment, as revealed by co-immunoprecipitation. (C) Co-immunoprecipitation and western blotting analysis identify the interaction of TXNIP with NLRP3. IP, immunoprecipitation; input of cell extract without immunoprecipitation ensures equal loading. (D) Effect of MitoQ on mtROS production in peritoneal macrophages. Results are expressed as mean ±SE. n= 5. *P <0.01.
Dashdorj et al. BMC Medicine 2013 11:178 doi:10.1186/1741-7015-11-178