Pluripotency-related gene expression by quantitative (q)PCR. Gene expression levels were normalized against 18S rRNA. The ratio of the relative expression for each gene to 18S was calculated by using the 2-ΔΔCq formula. hiPSC data were used as a calibrator for the results presented (100%). (A) OCT4 and KLF4; (B) NANOG, SOX2 and REX1.
Belmar-Lopez et al. BMC Medicine 2013 11:139 doi:10.1186/1741-7015-11-139