Pinocembrin inhibited mitochondrion-induced apoptosis. (A) Fluorescent images of B cell lymphoma 2 (Bcl-2), cytochrome c and nucleus in the same field. (B,C) Values of Mean_AvgInten of Bcl-2 and cytochrome c collected from the respective fluorescent channels using the same optical field. Data are expressed as mean ± SEM, n = 6, ***P < 0.001 vs control, ##P < 0.01, ###P < 0.001 vs copper. (D) Activity of caspase 3 and caspase 9 of human neuroblastoma SH-SY5Y cells overexpressing the Swedish mutant form of human APP (APPsw) in the presence of copper. Data are expressed as mean ± SEM, n = 4, ***P < 0.001 vs control, ##P < 0.01, ###P < 0.001 vs copper.
Liu et al. BMC Medicine 2012 10:105 doi:10.1186/1741-7015-10-105