Additional file 3.

Snail and NICD regulate invasion. (A-C) MEFs (A), Huh7 (B), and Hep3B (C) were infected by MSCV-NICD and/or MSCV-Snail, selected in puromycin, and analyzed for Notch1 and Snail expression by immunoblot with the indicated antibodies. β-actin served as an internal control. (D) Hep3B cells were transfected by Notch1 and/or Snail siRNA, treated with 300 μM H2O2 for 72 h, and analyzed for Notch1 and Snail expression by immunoblot with the indicated antibodies. E-cadherin, which is a Snail target gene, served as a marker of Snail activity. β-actin served as an internal control. (E) Hep3B cells were transfected by Notch1 and/or Snail siRNA, treated with 300 μM H2O2 for 72 h, and analyzed for Notch1 and Snail expression by immunoblot with the indicated antibodies. β-actin served as an internal control.

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Lim et al. BMC Biology 2011 9:83   doi:10.1186/1741-7007-9-83