Additional file 4.

Supplementary Figure S4. Two-hybrid analysis of Ulp1(3)C580S and Ulp1(3)C580A (isolates 1A and 3A) with small ubiquitin-like modifier (SUMO)/Smt3-BD. The presence of both Smt3 (pOBD2/TRP1) and Ulp1 constructs (pOAD/LEU2) was confirmed by growth on growth media lacking tryptophan and leucine (right plate). The interaction between the indicated Ulp1 constructs and Smt3 is shown as triplicate patches of cells on media lacking adenine (left plate).

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Elmore et al. BMC Biology 2011 9:74   doi:10.1186/1741-7007-9-74